CTNNB1 antibody (pTyr654) (Biotin)

Catalog No. ABIN126741

The Biotin-conjugated Mouse Monoclonal anti-CTNNB1 antibody (Clone 1B11) (ABIN126741) specifically detects CTNNB1 in WB. The antibody is reactive with Human, Mouse and Dog samples.

Quick Overview for CTNNB1 antibody (pTyr654) (Biotin) (ABIN126741)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reactivity: Human, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTNNB1 antibody is conjugated to Biotin

Application: Western Blotting (WB)

Clone: 1B11

Product Details anti-CTNNB1 Antibody (Biotin)

Binding Specificity: pTyr654

Specificity: This antibody specifically recognizes β-catenin phosphorylated at tyrosine 654 at 90 kDa and gamma-catenin at 70 kDa. Due to sequence homologies of the phospho-epitope surrounding pY 654 there may be crossreactions with other proteins (e.g. Annexin A11 at 56 kDa, gamma-catenin at 70 kDa, Desmoplakin at 81 kDa, TNFalpha-induced protein 2 at 73 kDa, Ribosomal protein S6 kinase alpha 6 at 90 kDa).

Characteristics: Synonyms: CTNNB1, CTNNB, beta-1 Catenin, beta catenin, beta-Catenin 1, Catenin-beta 1

Purification: Size exclusion chromatography

Components: incl. positive Control

Immunogen: Synthetic phosphopeptide conjugated to KLH.

Isotype: IgG1

Application Details

Application Notes: Western Blot. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS/0.09 % Na-Azide/PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody (aliquote in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Alternative Name: Catenin beta-1

Background: The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. Additionally, β-catenin is a substrate of both receptor and non-receptor tyrosine kinases. Tyrosine 86 and tyrosine 654 are substrates of EGF receptor and src family kinases while tyrosine 142 is a substrate of fer tyrosine kinases.Synonyms: Beta-catenin, CTNNB, CTNNB1

Gene ID: 1499

UniProt: P35222

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2