ENOS antibody (pSer1177)

Catalog No. ABIN126844

This Mouse Monoclonal antibody specifically detects ENOS in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for ENOS antibody (pSer1177) (ABIN126844)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENOS antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 1-50E-003

Product Details anti-ENOS Antibody

Binding Specificity: pSer1177

Specificity: This antibody specifically recognizes eNOS phosphorylated at Serine 1177.

Characteristics: Synonyms: Endothelial NOS, EC-NOS, NOS type III, Constitutive NOS, eNOS, Nitric oxide synthase,endothelial

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography

Immunogen: Synthetic phosphopeptide conjugated to KLH

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from pervanadate treated Bend3 cells. ELISA: 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 50 % Glycerol, PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: NOS3

Background: Endothelial NO synthase (eNOS) produces nitric oxid (NO) which plays a crucial role in cardiovascular homeostasis. Activation of the enzyme and NO production can be induced by phosphorylation of eNOS at serine 1177 by activated Akt/PKB.Synonyms: Constitutive NOS, EC-NOS, Endothelial NOS, NOS type III, Nitric oxide synthase, eNOS, endothelial

Gene ID: 4846

UniProt: P29474

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling