His Tag antibody

Catalog No. ABIN126947

The Mouse Monoclonal anti-His Tag antibody is suitable to detect His Tag. It has been validated for WB, IP and IF.

Quick Overview for His Tag antibody (ABIN126947)

Target: His Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: BV-G020

Product Details anti-His Tag Antibody

Sequence: HHHHHH

Characteristics: Synonyms: 6xHis-Tag, His Tag, HIS6 Tag, HHHHHH Tag

Isotype: IgG2b

Application Details

Application Notes: Western Blot: 1-2 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunocytochemistry: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol, 1 % BSA as stabilizer and 0.02 % Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or (in aliquots) at -70 °C for long term storage . Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Alternative Name: 6xHistidine Epitope Tag

Target Type: Tag

Background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification.