Keyhole Limpet Hemocyanin (KLH) antibody

Antigen: Keyhole Limpet Hemocyanin (KLH)

Clonality: Monoclonal (12B4-G3-A8)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129511

Additional Information

Immunogen: A BALB/c mouse was immunized with KLH (Keyhole Limpet Hemocyanin)

Format: Liquid (sterile filtered)

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 12B4-G3-A8

Description: KLH (Keyhole Limpet Hemocyanin) is a copper-containing oxygen carrier occurring freely dissolved in the hemolymph of many mollusks and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.

Specificity: This protein A purified mouse monoclonal antibody reacts specifically with KLH.

Application Details

Application Notes: ELISA 1:20,000 - 1:100,000. WESTERN BLOT 1:2,000 - 1:10,000. IMMUNOHISTOCHEMISTRY 1:1,000 - 1:5,000

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Purity: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide

Purification: Protein A purified

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage.Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product isstable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date ofreceipt.Application(s): Suitable for most immunological techniques requiring high titer binding and lot-to-lot consistency.

Research Area: Tags/Labels

Restrictions: For Research Use only