Keyhole Limpet Hemocyanin (KLH) antibody

Details for Product No. ABIN93503
Antigen
Reactivity
Megathura crenulata (Great Keyhole Limpet)
45
26
16
4
4
1
Host
Rabbit
54
28
13
1
Clonality
Polyclonal
Conjugate
Un-conjugated
9
8
7
4
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunolocalization (IL), ELISA, Western Blotting (WB)
56
35
13
7
5
3
3
3
3
1
1
1
1
1
1
1
1
Options
Immunogen purified keyhole limpet hemocyanin (KLH), whole molecule,
Isotype IgG
Predicted Reactivity Megathura crenulata - most commonly used carrier protein
Characteristics Expected / apparent Molecular Weight of the Antigene: Aprox. 400 kDa
Purification serum
Antigen
Background Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host's immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.
Molecular Weight ca. 400 kDa/subunit
UniProt Q6KC56
Application Notes Recommended Dilution: 1 : 10 000 (ELISA), 1 : 10 000 (WB), 1 : 1000 (IL).
Comment

Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE.

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution please add 50 µL of dest. water
Buffer PBS pH 7.4
Handling Advice Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Keyhole Limpet Hemocyanin (KLH) antibody (ABIN93503) anti-Keyhole Limpet Hemocyanin (KLH) antibody (Image 1)
Image no. 2 for anti-Keyhole Limpet Hemocyanin (KLH) antibody (ABIN93503) anti-Keyhole Limpet Hemocyanin (KLH) antibody (Image 2)