KLH antibody

Catalog No. ABIN103377

Product Details anti-KLH Antibody

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Reactivity: Keyhole Limpet

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLH antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Sterility: Sterile filtered

Immunogen: KLH (Keyhole Limpet Hemocyanin)
Immunogentype:Native

Isotype: IgG

Application Details

Application Notes: Anti-KLH Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-KLH antibody (ABIN103377)

Datta, Silverman, Phipps, Hiraragi, Ratner, Lairmore: "Human T-lymphotropic virus type-1 p30 alters cell cycle G2 regulation of T lymphocytes to enhance cell survival." in: Retrovirology, Vol. 4, pp. 49, (2007) (PubMed).

Target Details for KLH

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Abstract: KLH Products

Background: KLH Antibody detects Keyhole Limpet Hemocyanin protein which is a very large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000. KLH readily forms aggregates with molecular weights of 4,500,000-13,000,000 daltons. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata. KLH is being tested in a variety of cancer vaccines, including non-Hodgkins lymphoma, cutaneous melanoma, breast and bladder cancer.
Synonyms: Keyhole Limpet Hemocyanin, KLH, Anti-KLH antibody