Centromere Protein E (CENPE) antibody

Antigen: Centromere Protein E (CENPE)

Synonyms: KIF10, Kif10, 312kDa, CENP-E, AU019344, BC049989, C530022J18

Clonality: Polyclonal

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129515

Additional Information

Alternative name: CENP-E

Gene ID: NP_001804

Swiss-Prot: Q02224

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a full lengthrecombinant protein corresponding to human CENP-E protein. Hybridoma: Produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology. Protein sequence: Human CENP-E, 2701 amino acids, predicated MW 316.4 kDa. 1 maeegavavc vrvrplnsre eslgetaqvy wktdnnviyq vdgsksfnfd rvfhgnettk 61 nvyeeiaapi idsaiqgyng tifaygqtas gktytmmgse dhlgviprai hdifqkikkf 121 pdrefllrvs ymeiynetit dllcgtqkmk pliiredvnr nvyvadltee vvytsemalk 181 witkgeksrh ygetkmnqrs srshtifrmi lesrekgeps ncegsvkvsh lnlvdlagse 241 raaqtgaagv rlkegcninr slfilgqvik klsdgqvggf inyrdskltr ilqnslggna 301 ktriictitp vsfdetltal qfastakymk ntpyvnevst deallkryrk eimdlkkqle 361 evsletraqa mekdqlaqll eekdllqkvq nekienltrm lvtsssltlq qelkakrkrr 421 vtwclgkink mknsnyadqf niptnittkt hklsinllre idesvcsesd vfsntldtls … 2281 dieklkngiq kendricqvn nffnnriiai mnestefeer satiskeweq dlkslkekne 2341 klfknyqtlk tslasgaqvn pttqdnknph vtsratqltt ekirelensl heakesamhk 2401 eskiikmqke levtndiiak lqakvhesnk clektketiq vlqdkvalga kpykeeiedl 2461 kmklvkidle kmknakefek eisatkatve yqkevirllr enlrrsqqaq dtsvisehtd 2521 pqpsnkpltc gggsgivqnt kalilksehi rlekeisklk qqneqlikqk nellsnnqhl 2581 snevktwker tlkreahkqv tcenspkspk vtgtaskkkq itpsqckern lqdpvpkesp 2641 kscffdsrsk slpsphpvry fdnsslglcp evqnagaesv dsqpgpwhas sgkdvpeckt 2701 q

Format: Liquid (sterile filtered)

Isotype: IgG1  (Matching secondary antibodies)

Description: CENP-E, Centrosome-associated proteinE, is a kinesin-like minus-end directed microtubule motorprotein that accumulates in the G 2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is notpresent during interphase and first appears at thecentromere region of chromosomes during prometa-phase. CENP-E is proposed to be one of the motorsresponsible for mammalian chromosome movementand/or spindle elongation. CENP-E interacts with CENP-Fand BUBR1 kinase. CENP-E associates withkinetochores during congression, relocates to the spindlemidzone at anaphase, and is quantitatively discarded atthe end of the cell division.

Specificity: This Protein A purified antibody is directed against human CENP-E protein. The product waspurified from tissue culture supernatant by chromatography. This antibody reacts with CENP-E from human cells.Reactivity against homologues from other sources is not known.

Application Details

Application Notes: Recommended Dilutions: WESTERN BLOT 1:500 - 1:2,000 IMMUNOPRECIPITATION 1:200 IF MICROSCOPY 1:500 - 1:2,000 FLOW CYTOMETRY User Optimized. NoteThis protein A purified antibodyhas been tested for use in immunoprecipitation,immunofluorescence staining and western blot and iscapable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the enduser. Expect a predominant band at ~ 320 kDacorresponding to full-length protein by western blotting inthe appropriate cell lysate or extract.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) yearfrom date of opening.

Restrictions: For Research Use only

Images

Immunofluorescence microscopy. antibodies-online's monoclonal anti-CENPE antibody was used to detect CENPE protein visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A). Interphase cells show no discrete staining (bottom left, panel B). HeLa cells were fixed in paraformaldehyde and stained using this primary antibody. AlexaFluor 555