SPANX Family, Member C (SPANXC) antibody

Antigen: SPANX Family, Member C (SPANXC)

Synonyms: C, CTp11, CT11.3, SPANX-C

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129534

Additional Information

Alternative name: SPANX -C

Gene ID: CAD29839, CAB75344, CAI41662

Swiss-Prot: Q9NY87

Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length recombinant human SPANX-C protein.

Format: Liquid (sterile filtered)

Description: Human Sperm Proteins Associated with the Nucleus on X-chromosome (SPANX) are relatively low molecular weight cytoplasmic proteins found in testis and sperm. Their expression in other tissues indicates malignancies. Family members are observed as proteins that range from 15 to 20 kDa.

Specificity: This Protein A purified antibody is directed against human SPANX-C protein. The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above. A protein-protein BLAST analysis was used to suggest cross reactivity with most SPANX proteins from human, gorilla and chimpanzee. Reactivity against homologues from is not known.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:5,000 - 1:20,000 WESTERN BLOT 1:1,000 - 1:5,000 IMMUNOHISTOCHEMISTRY 1.25-2.5 ug/ml OTHER APPLICATIONS User Optimized. NoteThis protein A purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15-20 kDa in size corresponding to SPANX family members by western blotting in the appropriate human tissue. Most human cell lines are SPANX negative.

Concentration: 5.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to opening. Reconstitute with 0.1 ml deionized water (or equivalent). Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's Protein A purified anti-SPANX-C antibody shows detection of a band at ~17 kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000. IRDye

Immunohistochemistry. antibodies-online's affinity purified anti-SPANX-C antibody was used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human sperm and spermatids at 60X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.