Inhibitor of Growth Family, Member 1 (ING1) antibody

Antigen: Inhibitor of Growth Family, Member 1 (ING1)

Synonyms: p33, p47, p33ING1, p24ING1c, p33ING1b, p47ING1a, p33ING1c, ING1, MGC136918, zgc:136918

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129550

Additional Information

Alternative name: p33 (ING1)

Gene ID: NP_005528

Swiss-Prot: Q9UK53

Immunogen: This affinity purified antibody was prepared from whole Goat serum produced by repeated immunizations with asynthetic peptide corresponding aa 285-296 of Human p33 ING1 protein (Inhibitor of growth family, member 1). This sequenceshows 100% homology to all splice variants for ING1.

Format: Liquid (sterile filtered)

Description: P33 ING1 is a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encodedprotein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53-signalingpathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively splicedtranscript variants encoding distinct isoforms have been reported. The accession number listed below is for variant (4) that encodesthe longest isoform (D). Other synonyms for p33 ING1 include p33, p47, p33ING1, p24ING1c, p33ING1b, p47ING1a, growthinhibitor ING1, inhibitor of growth 1, tumor suppressor ING1 and growth inhibitory protein ING1.

Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizingpeptide after immobilization to a solid phase. Reactivity occurs against human p33 ING1 protein and is 100% homologous to all fourisoforms (A, B, C, and D) on record for this protein. Cross reactivity with p33 ING1 protein from mouse and rat sources is expectedbased on high homology (one amino acid change in sequence) by BLAST. Reactivity with p33 ING1 proteins from other sources isnot known.

Application Details

Application Notes: This affinity-purified antibody has been tested for use in ELISA and western blotting using over expressed celllysates. Expect a band approximately 47 kDa, 33 kDa or 24 kDa in size corresponding to variants of ING1 by western blotting in theappropriate cell lysate or extract. Specific conditions for other applications should be optimized by the end user. Dilutions: ELISA 1:4,000 - 1:14,000 WESTERN BLOT 1:500 - 1:3,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contentsand freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

Western blot analysis is shown using antibodies-online's Affinity Purified anti-p33 ING1 antibody to detect over expressed Human ING1 present in cell nuclear extracts. This western blot shows reactivity with purified recombinant GST tagged human ING1b (lane 1) and ING1c protein (lane 2). No reactivity is seen against GST or other ING proteins (not shown). Reactivity of this polyclonal antibody is similar to the specificity of a positive control monoclonal antibody (not shown). The blot was incubated with a 1:1,000 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Xiaolan Feng, U. Calgary.