Ubiquitin-Conjugating Enzyme E2N (UBE2N) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2N (UBE2N)

Synonyms: UBC13, AL022654, BB101821, 1500026J17Rik, MGC93937, ube2n, MGC64428, zgc:55726, wu:fb11b03, wu:fc08b06, UBE2N, MGC139855, ubc13, MGC75672, ubch-ben

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129560

Additional Information

Alternative name: Ubiquitin-Conjugating Enzyme E2N (UBC13)

Gene ID: NP_003339

Swiss-Prot: P61088

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizationswith a synthetic peptide corresponding to amino acids 40-51 of human UBC13 protein.

Format: Liquid (sterile filtered)

Description: UBC13 is a member ofthe E2 ubiquitin-conjugating enzyme family alsoknown as Ubiquitin-conjugating enzyme E2 N,Ubiquitin-protein ligase N, Ubiquitin carrier proteinN and Bendless-like ubiquitin-conjugating enzyme.The modification of proteins with ubiquitin is animportant cellular mechanism for targetingabnormal or short-lived proteins for degradation.Ubiquitination involves at least three classes ofenzymes: ubiquitin-activating enzymes, or E1s,ubiquitin-conjugating enzymes, or E2s, andubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. UBC13 forms a heterodimer with UBE2V2 whichthen catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. UBC13 also mediates transcriptional activationof target genes and plays a role in the control of progress through the cell cycle and differentiation, as well as error-freeDNA repair. UBC13 also contributes to the survival of cells after DNA damage.

Specificity: This affinity-purified antibody is directed against human UBC13 protein. The product wasaffinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggestcross reactivity with UBC13 protein from a broad range of sources including human, dog, bovine, Xenopus laevis,Xenopus tropicalis, , zebrafish, rat, mouse, chimpanzee, macaque and chicken based on 100% homology with theimmunizing sequence. Reactivity against homologues from other sources is not known.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blot. Specificconditions for reactivity should be optimized by the end user. Expect a band approximately 17 kDa in sizecorresponding to UBC13 protein by western blotting in the appropriate tissue or cell lysate or extract.

Concentration: 1.25 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior toopening. Dilute only prior to immediate use. Forextended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing andthawing. Expiration date is one (1) year from dateof opening.

Research Area: Proteases, Amino Acids

Restrictions: For Research Use only

Images

Western blot using antibodies-online's affinity purified anti-UBC13 antibody shows detection of UBC13 protein in human small intestine lysate (lane 1) but not in mouse thymus lysate (lane 2). The heavily stained band in lane 1 (arrowhead) indicates this particular gel was overloaded with protein. The identity of minor reactive bands is unknown but could represent E2 complexes. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:10,000 dilution of Alexa Fluor