F-Box Protein 5 (FBXO5) antibody

Antigen: F-Box Protein 5 (FBXO5)

Synonyms: EMI1, FBX5, Fbxo31, Emi1, C85305, 2510044I10Rik, fc65h02, MGC136397, MGC152677, MGC158541, MGC162839, wu:fc65h02, wu:fe06e07, wu:fz79f03, zgc:136397, zgc:158541, FBXO5, MGC148774, fbxo5, Fbxo5, ACYPI007854

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129601

Additional Information

Alternative name: Fbp5A (F-Box Protein)

Gene ID: NP_036309

Swiss-Prot: Q9UKT4

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human Fbp5A protein.

Format: Liquid (sterile filtered)

Description: Fbp5A is a member of the F-box protein family that is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to Xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase-promoting complex.

Specificity: This affinity-purified antibody is directed against human Fbp5A protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Fbp5A protein from human and chimpanzee based on 100% homology with the immunizing sequence. Expect partial reactivity with Fbp5A from dog based on partial (~92%, 13/14) sequence homology. Reactivity against homologues from other sources is not known.

Synonyms: EMI1, FBX5, Fbxo31, Emi1, C85305, 2510044I10Rik, fc65h02, MGC136397, MGC152677, MGC158541, MGC162839, wu:fc65h02, wu:fe06e07, wu:fz79f03, zgc:136397, zgc:158541, FBXO5, MGC148774, fbxo5, Fbxo5, ACYPI007854

Application Details

Application Notes: Recommended Dilutions: ELISA 1:5,000 - 1:20,000 WESTERN BLOT 1:500 - 1:3,000 IMMUNOHISTOCHEMISTRY 1:500 - 1:3,000 OTHER APPLICATIONSS User Optimized. Note: This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 51 kDa in size corresponding to Fbp5A protein by western blotting in the appropriate cell lysate or extract.

Concentration: 1.3 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's affinity purified anti-Fbp5A antibody shows detection of a major band corresponding to Fbp5A protein in a human HeLa whole cell lysate (lane 1 arrowhead). The identity of cross-reactive minor bands at ~72 kDa and 150 kDa is unknown. Specific band staining is blocked when the antibody is preincubated with the immunizing peptide (lane 2). Approximately 33 ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose. The membrane was blocked using BLOTTO. Primary antibody was used at a 1:500 dilution in BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of IRDye