FBXO5 antibody (AA 1-250)

Catalog No. ABIN3024715

The Mouse Monoclonal anti-FBXO5 antibody (Clone EMI1-1176) (ABIN3024715) specifically detects FBXO5 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for FBXO5 antibody (AA 1-250) (ABIN3024715)

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FBXO5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EMI1-1176

Product Details anti-FBXO5 Antibody

Binding Specificity: AA 1-250

Purpose: EMI1 Antibody

Purification: Protein G affinity chromatography

Immunogen: A recombinant fragment (203 amino acid residues between aa 1-250) from the human protein was used as the immunogen for the EMI1 antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the EMI1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the EMI1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for FBXO5

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Alternative Name: EMI1

Background: Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

UniProt: Q9UKT4

Pathways: Mitotic G1-G1/S Phases