Fanconi Anemia Group A Protein (FANCA) antibody

Antigen: Fanconi Anemia Group A Protein (FANCA)

Synonyms: FA, FA1, FAA, FAH, FA-H, FACA, FANCH, MGC75158

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Chimpanzee

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN129606

Additional Information

Alternative name: FANCA (Fanconi Anemia Complementation Group A)

Swiss-Prot: O15360

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 995-1009 of human FANCA protein.

Format: Liquid (sterile filtered)

Description: FANCA (also called Protein FACA or Fanconi anemia group A protein) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCA may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, and FANCL. The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity, they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group A. Alternative splicing results in multiple transcript variants encoding different isoforms. Variant 1 (isoform a) encodes the longest transcript. Variant 2 (isoform b) contains an alternate exon, which results in an early stop codon, compared to variant 1. Isoform b has a shorter C-terminus when compared to isoform a. Mutations in this gene are the most common cause of Fanconi anemia.

Specificity: This affinity-purified antibody is directed against human FANCA protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with FANCA protein from human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a main band at approximately 163 kDa in size corresponding to FANCA by western blotting in the appropriate cell lysate or extract or human tissue. Dilutions: ELISA 115,000 - 160,000 WESTERN BLOT 1500 - 13,000 IF MICROSCOPY User Optimized OTHER APPLICATION User Optimized

Concentration: 1.45 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's affinity purified anti- FANCA antibody shows detection of FANCA only in FANCA transfected GM6914 cell lysates. No staining is seen in lysates prepared from FANCA (-/-) cells in the absence of FANCA transfection. Modified from Smogorzewska et al (2007) Cell 129, 289-301.