Fanconi Anemia Complementation Group G (FANCG) antibody

Antigen: Fanconi Anemia Complementation Group G (FANCG)

Synonyms: FAG, XRCC9, fancg

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129607

Additional Information

Alternative name: FANCG (Fanconi Anemia Complementation Group G)

Gene ID: NP_004620

Swiss-Prot: O15287

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1-12 of human FANCG protein. Protein Sequence Human FANCG, 622 aa, predicted MW 68.6 kDa 1 msrqttsvgs scldlwrekn drlvrqakva qnsgltlrrq qlaqdalegl rgllhslqgl 61 paavpvlple ltvtcnfiil raslaqgfte dqaqdiqrsl ervletqeqq gprleqglre 121 lwdsvlrasc llpellsalh rlvglqaalw lsadrlgdla llletlngsq sgaskdllll 181 lktwsppaee ldapltlqda qglkdvllta fayrqglqel itgnpdkals slheaasglc 241 prpvlvqvyt algschrkmg npqrallylv aalkegsawg pplleasrly qqlgdttael 301 eslellveal nvpcsskapq flievelllp ppdlasplhc gtqsqtkhil asrclqtgra 361 gdaaehyldl lallldssep rfspppsppg pcmpevflea avaliqagra qdaltlceel 421 lsrtssllpk msrlwedark gtkelpycpl wvsathllqg qawvqlgaqk vaisefsrcl 481 ellfratpee keqgaafnce qgcksdaalq qlraaalisr glewvasgqd tkalqdflls 541 vqmcpgnrdt yfhllqtlkr ldrrdeatal wwrleaqtkg shedalwslp lylesylswi 601 rpsdrdafle efrtslpksc dl

Format: Liquid (sterile filtered)

Description: FANCG (also called Protein FACG or DNA-repair protein XRCC9) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCG may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. This protein may also function as a tumor suppressor gene. FANCG belongs to the multi-subunit Fanconi Anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. FANCG contains a 5-prime GC-rich untranslated region characteristic of housekeeping genes. The putative 622-amino acid protein has a leucine-zipper motif at its N-terminus. FANCG is mainly found within the nucleus although some protein is localized in the cytoplasm. This protein is highly expressed in testis and thymus and is also found in lymphoblasts. Fanconi anemia is an autosomal recessive disorder with diverse clinical symptoms, including developmental anomalies, bone marrow failure, and early occurrence of malignancies caused by defects in the FANCG gene. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.

Specificity: This affinity-purified antibody is directed against human FANCG protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with FANCG protein from human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 69 kDa in size corresponding to FANCG by western blotting in the appropriate human tissue. Dilutions: ELISA 1:10,000 - 1:50,000 WESTERN BLOT 1:500 - 1:2,000 IMMUNOHISTOCHEMISTRY 1:500 - 1:2,000 OTHER APPLICATIONSS User Optimized

Concentration: 0.93 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's affinity purified anti-FANCG antibody shows detection of a band at ~69 kDa (arrowhead) corresponding to FANCG present in a HeLa whole cell lysate. Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4