Fanconi Anemia, Complementation Group C (FANCC) antibody

Antigen: Fanconi Anemia, Complementation Group C (FANCC)

Synonyms: Facc, BB116513, FANCC, zgc:154105, fancc, FA3, FAC, FACC, FLJ14675

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129608

Additional Information

Alternative name: FANCC (Fanconi Anemia Complementation Group C)

Gene ID: NP_000127

Swiss-Prot: Q00597

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with asynthetic peptide corresponding to amino acids 96-112 of Human FANCC.

Format: Liquid (sterile filtered)

Description: FANCC (also called Protein FACC or Fanconi AnemiaGroup C protein) is involved in DNA repair,perhaps specifically with post-replicationrepair or a cell cycle checkpoint function.FANCC may also be implicated in interstrandDNA cross-link repair and in the maintenanceof normal chromosome stability. FANCCbelongs to the multi-subunit Fanconi Anemia(FA) complex composed of FANCA, FANCB,FANCC, FANCE, FANCF, FANCG,FANCL/PHF9 and FANCM. FANCC is mainlyfound within the nucleus although someprotein is localized in the cytoplasm. Thisprotein is ubiquitously expressed. Defects inFANCC are a cause of Fanconi anemia (FA).FA is a genetically heterogeneous, autosomalrecessive disorder characterized byprogressive pancytopaenia, a diverseassortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.

Specificity: This affinity-purified antibody is directed against human FANCC protein. The product was affinitypurified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivitywith FANCC protein from human and chimpanzee based on 100% homology with the immunizing sequence. Reactivityagainst homologues from other sources is not known.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditionsfor reactivity should be optimized by the end user. Expect a band approximately 63 kDa in size corresponding to FANCC bywestern blotting in the appropriate human tissue. Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditionsfor reactivity should be optimized by the end user. Expect a band approximately 63 kDa in size corresponding to FANCC bywestern blotting in the appropriate human tissue. Dilutions: ELISA 1:20,000 - 1:80,000 WESTERN BLOT 1:1,000 - 1:3,000 IF MICROSCOPY User Optimized OTHER APPLICATION User Optimized

Concentration: 1.45 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date ofopening.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's affinity purified anti-FANCC antibody shows detection of a band at ~63 kDa (arrowhead) corresponding to FANCC present in a HeLa whole cell lysate. The identity of the lower molecular weight band is unknown. Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDSPAGE. After blocking, the membrane was probed overnight at 4