Early Growth Response 1 (EGR1) antibody

Antigen: Early Growth Response 1 (EGR1)

Synonyms: TIS8, AT225, G0S30, NGFI-A, ZNF225, KROX-24, ZIF-268, EGR-1, Egr1, egr, Zenk, Egr-1, NGFIA, Zfp-6, ETR103, Krox-1, Krox24, NGF1-A, Zif268, Krox-24, A530045N19Rik, Ngf1, Ngfi, zif-268, EGR1, MGC139820

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129622

Additional Information

Alternative name: Early Growth Response Protein-1 (EGR-1)

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizationswith a synthetic peptide corresponding to amino acids 94-108 of Human EGR-1. Protein Sequence: Human EGR-1, 543 aa, predicted MW 57.5 kDa 1 maaakaemql msplqisdpf gsfphsptmd nypkleemml lsngapqflg aagapegsgs 61 nssssssggg ggggggsnss sssstfnpqa dtgeqpyehl taesfpdisl nnekvlvets 121 ypsqttrlpp itytgrfsle papnsgntlw peplfslvsg lvsmtnppas sssapspaas 181 sasasqsppl scavpsndss piysaaptfp tpntdifpep qsqafpgsag talqypppay 241 paakggfqvp mipdylfpqq qgdlglgtpd qkpfqglesr tqqpsltpls tikafatqsg 301 sqdlkalnts yqsqlikpsr mrkypnrpsk tppherpyac pvescdrrfs rsdeltrhir 361 ihtgqkpfqc ricmrnfsrs dhltthirth tgekpfacdi cgrkfarsde rkrhtkihlr 421 qkdkkadksv vassatssls sypspvatsy pspvttsyps pattsypspv ptsfsspgss 481 typspvhsgf pspsvattys svppafpaqv ssfpssavtn sfsastglsd mtatfsprti 541 eic

Format: Liquid (sterile filtered)

Description: EGR-1 (also called Early Growth Response protein 1, Krox-24 protein, ZIF268, Nerve growth factor-induced protein A or NGFI-A, Transcription factor ETR103, andZinc finger protein 225 or AT225) is a transcriptional regulatorthat recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site). EGR-1 activates thetranscription of target genes whose products are required formitogenesis and differentiation. EGR-1 is a nuclear proteininduced by growth factors. Expression has been identified in avariety of cancers.

Specificity: This affinity purified antibody is directed againsthuman EGR-1 protein. The product was affinity purified frommonospecific antiserum by immunoaffinity purification. A BLASTanalysis was used to suggest reactivity with this protein from human andchimpanzee sources based on 100% homology for the immunogensequence. This antibody is expected to cross react with EGR-1

Application Details

Application Notes: Recommended Dilutions: ELISA 1:4,000 - 1:16,000 WESTERN BLOT 1:500 - 1:3,000 IMMUNOHISTOCHEMISTRY 2 ug/ml to 20 ug/ml OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested foruse in ELISA, immunohistochemistry and western blot. Specificconditions for reactivity should be optimized by the end user. Expect aband at ~58 kDa in size corresponding to EGR-1 by western blotting inthe appropriate cell lysate or extract.

Concentration: 1.46 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) yearfrom date of opening.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's Affinity Purified anti-EGR-1 antibody shows detection of a predominant band at ~58 kDa corresponding to EGR-1 present in mouse embryonic fibroblast whole cell lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye

Immunohistochemistry. antibodies-online's Affinity Purified anti-EGR-1 antibody was used at a 10 ug/ml to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.