Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) antibody

Antigen: Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27)

Synonyms: APC3, HNUC, CDC27Hs, D0S1430E, D17S978E, wu:fb54g06, wu:fc88c12, AI452358, BC023187, MGC80529, CDC27, MGC145551, NV10210

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129644

Additional Information

Alternative name: CDC27

Gene ID: NP_001247

Swiss-Prot: P30260

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizationswith a synthetic peptide corresponding amino acids 422-430 of Human cdc27. Protein Sequence: Human Cdc27, 824 aa, predicted MW 91.9 kDa 1 mtvlqepvqa aiwqalnhya yrdavflaer lyaevhseea lfllatcyyr sgkaykayrl 61 lkghscttpq ckyllakccv dlsklaegeq ilsggvfnkq kshddivtef gdsacftlsl 121 lghvycktdr lakgsecyqk slslnpflws pfeslceige kpdpdqtfkf tslqnfsncl 181 pnscttqvpn hslshrqpet vltetpqdti elnrlnless nskyslntds svsyidsavi 241 spdtvplgtg tsilskqvqn kpktgrsllg gpaalspltp sfgilpletp spgdgsylqn 301 ytntppvidv pstgapskks varigqtgtk svfsqsgnsr evtpilaqtq ssgpqtsttp 361 qvlsptitsp pnalprrssr lftsdssttk enskklkmkf ppkipnrktk sktnkggitq 421 pnindsleit kldssiiseg kistitpqiq afnlqkaaaa glmsllremg kgylalcsyn 481 ckeainilsh lpshhyntgw vlcqigrayf elseymqaer ifsevrrien yrvegmeiys 541 ttlwhlqkdv alsvlskdlt dmdknspeaw caagncfslq rehdiaikff qraiqvdpny 601 ayaytllghe fvlteeldka lacfrnairv nprhynawyg lgmiyykqek fslaemhfqk 661 aldinpqssv llchigvvqh alkksekald tlnkaividp knplckfhra svlfanekyk 721 salqeleelk qivpkeslvy fligkvykkl gqthlalmnf swamdldpkg annqikeaid 781 krylpddeep itqeeqimgt desqessmtd addtqlhaae sdef

Format: Liquid (sterile filtered)

Description: Cdc27 (also known as cell division cycleprotein 27 homologue, CDC27Hs and H-NUC) sharesstrong similarity with Saccharomyces cerevisiae proteinCdc27, and the gene product of Schizosaccharomycespombe nuc 2. Cdc27 is a component of anaphase-promoting complex (APC), which is composed of eightprotein subunits and highly conserved in eukaryotic cells.APC catalyzes the formation of cyclin B-ubiquitin conjugatethat is responsible for the ubiquitin-mediated proteolysis ofB-type cyclins. This protein and 3 other members of theAPC complex contain the TPR (tetratricopeptide repeat), aprotein domain important for protein-protein interaction.This protein was shown to interact with mitotic checkpointproteins including Mad2, p55CDC and BUBR1, and thusmay be involved in controlling the timing of mitosis. Cdc27has a nuclear localization.

Specificity: This affinity purified antibody is directed against human Cdc27 protein. The product wasaffinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggestreactivity with this protein from human, chimpanzee, chicken, rat and dog based on 100% homology for the immunogensequence. Expect cross reactivity with cdc27 from mouse as only a single amino acid residue change is found for theimmunogen sequence. Cross reactivity with cdc27 homologues from other sources has not been determined.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:5,000 - 1:40,000 WESTERN BLOT 1:500 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specificconditions for reactivity should be optimized by the end user. Expect a band approximately 92 kDa in sizecorresponding to Cdc27 by western blotting in the appropriate cell lysate or extract.

Concentration: 1.95 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) yearfrom date of opening.

Research Area: CD Antigens, Surface Receptors of Immune Cells, Cell Cycle, Coagulation

Restrictions: For Research Use only