CDC27 antibody

Catalog No. ABIN1682927

The Rabbit Monoclonal anti-CDC27 antibody is suitable to detect CDC27 in samples from Human. It has been validated for WB.

Quick Overview for CDC27 antibody (ABIN1682927)

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CDC27 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDC27 Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Monoclonal Antibodies

Purification: Affinity purification

Immunogen: A synthesized peptide derived from human CDC27

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide,0.05 % BSA,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for CDC27

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Alternative Name: CDC27

Background: The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014],ANAPC3, APC3, CDC27Hs, D0S1430E, D17S978E, H-NUC, HNUC, NUC2,Cell Biology & Developmental Biology,Cell Cycle,Ubiquitin,Ubiquitin_Ubiquitin-Proteasome Signaling Pathway,CDC27

Molecular Weight: 91 kDa

Gene ID: 996

UniProt: P30260