Heat Shock Protein 90 (HSP90) antibody

Antigen: Heat Shock Protein 90 (HSP90)

Synonyms: swo1, hsp90, HSP90, htpG, SCBAC25F8.08, 23.m06066, 17.m07646, HSP90-1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129685

Additional Information

Alternative name: Heat Shock Protein 90

Swiss-Prot: P46633

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 289-300 of human Hsp90 protein. Protein Sequence: Human Hsp90, 732 aa, predicted MW 84.7 kDa 1 mpeetqtqdq pmeeeevetf afqaeiaqlm sliintfysn keiflrelis nssdaldkir 61 yetltdpskl dsgkelhinl ipnkqdrtlt ivdtgigmtk adlinnlgti aksgtkafme 121 alqagadism igqfgvgfys aylvaekvtv itkhnddeqy awessaggsf tvrtdtgepm 181 grgtkvilhl kedqteylee rrikeivkkh sqfigypitl fvekerdkev sddeaeeked 241 keeekekeek esedkpeied vgsdeeeekk dgdkkkkkki kekyidqeel nktkpiwtrn 301 pdditneeyg efyksltndw edhlavkhfs vegqlefral lfvprrapfd lfenrkkknn 361 iklyvrrvfi mdnceelipe ylnfirgvvd sedlplnisr emlqqskilk virknlvkkc 421 lelftelaed kenykkfyeq fskniklgih edsqnrkkls ellryytsas gdemvslkdy 481 ctrmkenqkh iyyitgetkd qvansafver lrkhgleviy miepideycv qqlkefegkt 541 lvsvtkegle lpedeeekkk qeekktkfen lckimkdile kkvekvvvsn rlvtspcciv 601 tstygwtanm erimkaqalr dnstmgymaa kkhleinpdh siietlrqka eadkndksvk 661 dlvillyeta llssgfsled pqthanriyr miklglgide ddptaddtsa avteempple 721 gdddtsrmee vd

Format: Liquid (sterile filtered)

Description: Hsp90 is a member of the heat shock protein 90 family that functions as a molecular chaperone and has ATPase activity. Hsp90 family proteins are highly conserved between isoforms and species. Several signal transduction pathways depend on Hsp90 function including erbB2, steroid hormone receptors (such as androgen, progesterone, glucocorticoid, and aryl-hydrocarbon), and hypoxia sensing (Hif1alpha). Recent reports show that tumor cells are more sensitive to Hsp90 inhibition and that Hsp90 from tumor cells is more sensitive to small molecule inhibitors (eg 17AAG). The mechanism of this differential sensitivity of normal versus tumor Hsp90 is not known (although mutation has been ruled out). One possible mechanism may be differences in post-translational modification of tumor Hsp90. Hsp90 is a cytoplasmic protein that forms a homodimer in vivo, and interacts with TOM34, AHSA1, HDAC6 and SMYD3.

Specificity: This affinity-purified antibody is directed against human Hsp90 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Hsp90 from human, mouse, rat, monkey, chicken and Drosophila based on 100% homology with the immunizing sequence. Reactivity of this antibody with Hsp90 from other species is unknown.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to Hsp90 protein by western blotting in the appropriate cell lysate or extract. Dilutions: ELISA 1:5,000 - 1:25,000 WESTERN BLOT 1:500 - 1:2,500 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 1.24 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. General

Research Area: Metabolism, Heat Shock Proteins

Restrictions: For Research Use only

Images

anti-Heat Shock Protein 90 (HSP90) antibody