HSP90AA1 antibody (AA 289-300)

Catalog No. ABIN129685

Supplier Product No.: 600-401-929

This Rabbit Polyclonal antibody specifically detects HSP90AA1 in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse, Rat, Monkey, Chicken and Drosophila melanogaster.

Quick Overview for HSP90AA1 antibody (AA 289-300) (ABIN129685)

Target: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Reactivity: Human, Mouse, Rat, Monkey, Chicken, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP90AA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-HSP90AA1 Antibody

Binding Specificity: AA 289-300

Supplier Product No.: 600-401-929

Supplier: Rockland

Purpose: HSP90 alpha Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against human Hsp90 protein.

Characteristics: Synonyms: rabbit anti-Heat Shock Protein HSP 90-alpha antibody, HSP 86 antibody, Heat shock 86 kDa antibody, Renal carcinoma antigen NY REN 38 antibody, Heat shock 90 kDa protein 1 alpha antibody, Lipopolysaccharide-associated protein 2, LAP-2, HSP90A, HSPC1, HSPCA, HSP90AA1, HSP 90-α

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 289-300 of human Hsp90 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to Hsp90 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.24 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HSP90AA1

Target: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Alternative Name: HSP90AA1

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Hsp90 is a member of the heat shock protein 90 family that functions as a molecular chaperone and has ATPase activity. Hsp90 family proteins are highly conserved between isoforms and species.  Several signal transduction pathways depend on Hsp90 function including erbB2, steroid hormone receptors (such as androgen, progesterone, glucocorticoid, and aryl-hydrocarbon), and hypoxia sensing (Hif1alpha).  Recent reports show that tumor cells are more sensitive to Hsp90 inhibition and that Hsp90 from tumor cells is more sensitive to small molecule inhibitors (eg 17AAG).  The mechanism of this differential sensitivity of normal versus tumor Hsp90 is not known (although mutation has been ruled out).  One possible mechanism may be differences in post-translational modification of tumor Hsp90.

Gene ID: 3320

NCBI Accession: NP_001017963

UniProt: P07900

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals