Endothelium antibody (Biotin)

Catalog No. ABIN133355

Product Details

Target: Endothelium

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Specificity: Rat Endothelium

Immunogen: Rat Peritoneal Macrophages . Fusion Partner: NSO/U . Immunocyte Donor: BALB/c Spleen

Clone: OX-43

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/ml

Buffer: Biotin conjugated Ig buffered in PBS, 0.02% NaN and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: Endothelium

Background: Anti-rat endothelium monoclonal antibody recognizes a surface protein of MW 90 kDa and generally reacts with all vascular endothelium in the rat except that of brain capillaries (1). This is the reciprocal tissue pattern to that of the transferrin receptor. It has been shown that the expression of ABIN133355is on the luminal surface of blood vessels. ABIN133355labels all peritoneal macrophages, a sub-population of alveolar macrophages (65%) and rare interstitial cells in the brain and heart (1). In addition, anti-rat endothelium mAb labels circulating erythrocytes, 22% of peripheral blood mononuclear cells and 17% of nucleated cells in bone marrow (1). ABIN133355does not label granulocytes, dendritic cells, lymphocytes, or lymphocyte blasts, thymocytes, lymph node cells, mast cells and platelets. ABIN133355has been invaluable in the demonstration of molecular heterogeneity of vascular endothelium.