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Details for Product No. ABIN135135

Integrin alpha M (ITGAM) antibody

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Antigen
Synonyms CR3, CR3A, MAC1, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik, CD11B, MAC-1, MAC1A, MO1A, SLEB6, C3bi
Reactivity
»Alternatives Mouse (Murine)
Host
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
Pubmed 4 references available
Catalog no. ABIN135135
Quantity 0.5 mg
Price
211.20 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen C57BL/10 mouse splenic T cells and concanavaline A-activated C57BL/10 splenocytes1
Clone M1-70
Isotype IgG2b
Characteristics The antibody detects: M subunit (Mr 170 kDa) of the CD11b/CD18 heterodimer
Cross-reaction of M1/70 to human CD11b has been observed on monocytes, polymorphonuclear leukocytes, and NK cells.
Purification Purified
Purity Purified
Alternative Name CD11b/Mac-1a
Background CD11b, the integrin M subunit, combines with CD18, the integrin 2 subunit, to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1/CD54. Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 expression is rapidly up-regulated on neutrophils after activation and in the same time period that CD62L is shed from the cell surface. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils.
Application Notes Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

In vitro and in vivo blocking

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0.
Preservative Azide free
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Springer, Galfrè, Secher et al.: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978 (PubMed).

Flotte, Springer, Thorbecke: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." in: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, 1983 (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994 (PubMed).

Gyetko, Sud, Kendall et al.: "Urokinase receptor-deficient mice have impaired neutrophil recruitment in response to pulmonary Pseudomonas aeruginosa infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 3, pp. 1513-9, 2000 (PubMed).

Alternatives for antigen "Integrin alpha M (ITGAM)", type "Antibodies"
Hosts (155), (124), (30), (4), (2)
Reactivities (156), (125), (25), (9), (6), (5), (4), (3), (3), (3), (1), (1), (1), (1)
Applications (251), (69), (44), (41), (36), (33), (27), (26), (13), (11), (9), (3), (3), (2), (1), (1), (1)
Conjugates (44), (32), (32), (10), (10), (8), (4), (4), (3), (2), (2), (2), (1), (1)
Epitopes (3), (3), (1), (1), (1), (1), (1), (1)
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