Integrin alpha M (ITGAM) antibody

Antigen: Integrin alpha M (ITGAM)

Synonyms: CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, MGC117044, CR3, MAC1, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik, ITGAM, Itgam

Clonality: Monoclonal (M1-70)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Blocking Antibody (Inhibition), Immunoprecipitation (IP)

Catalog no.: ABIN135141

Additional Information

Alternative name: CD11b/Mac-1a

Immunogen: C57BL/10 mouse splenic T cells and concanavaline A-activated C57BL/10 splenocytes1

Isotype: IgG2b

Clone: M1-70

Description: M subunit (Mr 170 kDa) of the CD11b/CD18 heterodimer CD11b, the integrin M subunit, combines with CD18, the integrin 2 subunit, to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1/CD54.2, 3 Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities.2-8 Mac-1 expression is rapidly up-regulated on neutrophils after activation and in the same time period that CD62L is shed from the cell surface. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils.4, 9, 10 Cross-reaction of M1/70 to human CD11b has been observed on monocytes, polymorphonuclear leukocytes, and NK cells.4,

Application Details

Application Notes: Working Dilutions: Flow Cytometry: Purified (1 ug/10^6 cells), Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), Phycoerythrin (0.3 ug/10^6 cells), Allophycocyanin (0.3 ug/10^6 cells), Spectral RedTM (0.3 ug/10^6 cells), Cyanine 5 (0.3 ug/10^6 cells), R-phycoerythrin-Cyanine 7 (0.3 ug/10^6 cells), Allophycocyanin-Cyanine 7 (0.3 ug/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Buffer: Low-endotoxin, azide-free (LEAF)

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The Spectral RedTM (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CYTM7), and Allophycocyanin-Cyanine 7 (APC-CYTM7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20C. The Cyanine 5 (CYTM5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Restrictions: For Research Use only

Publications

Springer, Galfrè, Secher et al.: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978 (PubMed).

Flotte, Springer, Thorbecke: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." in: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, 1983 (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994 (PubMed).

Gyetko, Sud, Kendall et al.: "Urokinase receptor-deficient mice have impaired neutrophil recruitment in response to pulmonary Pseudomonas aeruginosa infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 3, pp. 1513-9, 2000 (PubMed).