CD11b antibody

Catalog No. ABIN135141

The Rat Monoclonal anti-CD11b antibody (Clone M1-70) (ABIN135141) specifically detects CD11b in FACS, IP, IHC (fro), ICC, IHC (p) and BR. The antibody is reactive with Human, Mouse, Rabbit, Baboon and Rhesus Monkey samples.

Quick Overview for CD11b antibody (ABIN135141)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human, Mouse, Rabbit, Baboon, Rhesus Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Blocking Reagent (BR)

Clone: M1-70

Product Details anti-CD11b Antibody

Purpose: Rat Anti-Mouse CD11b-LE/AF

Specificity: Mouse/Human/Rhesus/Baboon/Rabbit CD11b, Mr 170 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD11b antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, and blocking assays.

Endotoxin Level: Low Endotoxin

Immunogen: C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background: CD11b, the integrin M subunit, combines with CD18, the integrin 2 subunit, to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1/CD54. Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 expression is rapidly up-regulated on neutrophils after activation and in the same time period that CD62L is shed from the cell surface. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils.

Gene ID: 16409, 3684, 714832

UniProt: P05555

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation