Ly6C antibody

Catalog No. ABIN135293

The Rat Monoclonal anti-Ly6C antibody has been validated for FACS. It is suitable to detect Ly6C in samples from Mouse. There is 1 publication available.

Quick Overview for Ly6C antibody (ABIN135293)

Target: Ly6C (Ly-6C) (Ly-6C)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Ly6C antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: HK1-4

Product Details anti-Ly6C Antibody

Specificity: Mouse Ly-6C, Mr 14-17 kDa

Characteristics: Rat Anti-Mouse Ly-6C-UNLB

Purification: Purified

Immunogen: L3 cloned CTL cells

Isotype: IgG2c

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , Activ - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PACBLU conjugate 0.3 g/106 cells PE conjugate 0.2 g/106 cells AF647 and AF700 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro activation of T lymphocytes

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-Ly6C antibody (ABIN135293)

Chen, Grimbaldeston, Tsai, Weissman, Galli: "Identification of mast cell progenitors in adult mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 32, pp. 11408-13, (2005) (PubMed).

Target Details for Ly6C

Target: Ly6C (Ly-6C) (Ly-6C)

Alternative Name: Ly-6C

Background: Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells, while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes.