Ly6C antibody

Catalog No. ABIN2191831

Product Details anti-Ly6C Antibody

Target: Ly6C (Ly-6C) (Ly-6C)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Ly6C antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoassay (IA), Detection (D)

Sterility: 0.2 μm filtered

Clone: ER-MP20

Application Details

Application Notes: For immunohistology and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-Ly6C antibody (ABIN2191831)

Manitz, Horst, Seeliger, Strey, Skryabin, Gunzer, Frings, Schönlau, Roth, Sorg, Nacken: "Loss of S100A9 (MRP14) results in reduced interleukin-8-induced CD11b surface expression, a polarized microfilament system, and diminished responsiveness to chemoattractants in vitro." in: Molecular and cellular biology, Vol. 23, Issue 3, pp. 1034-43, (2003) (PubMed).

Henkel, McKercher, Leenen, Maki: "Commitment to the monocytic lineage occurs in the absence of the transcription factor PU.1." in: Blood, Vol. 93, Issue 9, pp. 2849-58, (1999) (PubMed).

Chan, Leenen, Bertoncello, Nishikawa, Hamilton: "Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression." in: Blood, Vol. 92, Issue 4, pp. 1423-31, (1998) (PubMed).

de Bruijn, Slieker, van der Loo, Voerman, van Ewijk, Leenen: "Distinct mouse bone marrow macrophage precursors identified by differential expression of ER-MP12 and ER-MP20 antigens." in: European journal of immunology, Vol. 24, Issue 10, pp. 2279-84, (1994) (PubMed).

Target Details for Ly6C

Target: Ly6C (Ly-6C) (Ly-6C)

Abstract: Ly-6C Products

Synonyms: AA682074 antibody, AA959465 antibody, Ly-6C antibody, Ly-6C1 antibody, Ly6c antibody, lymphocyte antigen 6 complex, locus C1 antibody, Ly6c1 antibody

Background: The monoclonal antibody ER-MP20 specifically reacts with mouse macrophage precursor cells in the mid-stage of their development (late CFU-M, monoblasts and monocytes). The antigen is a 14 kD surface protein which is very similar to Ly-6C and may be analogous to human CD59. It is inducible by IFN-alpha, IFN-beta and IFN-gamma. In tissue sections, the antigen is found on macrophage precursor subpopulations. In the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Activated macrophages in inflammatory tissues also express the ER-MP20-related antigen. The monoclonal antibody ER-MP20 has been raised after immunization of rats with mouse macrophage cell lines and reacts with mouse macrophage precursor cells. The monoclonal antibody also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan- macrophage marker BM8 (anti-F4/80) is recommended. In combination with an anti-mouse CD31/PECAM-1 antibody, ER-MP20 can be used to evaluate the cellular composition in murine bone marrow (e.g. using flow cytometric analysis). ER-MP20 also detects a wide range of endothelial cells.