Integrin alpha M (ITGAM) antibody (PE)

Antigen: Integrin alpha M (ITGAM)

Synonyms: CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, MGC117044, CR3, MAC1, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik, ITGAM, Itgam

Clonality: Monoclonal (ICRF44)

Host: Mouse

Reactivity: Human

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN135738

Additional Information

Alternative name: CD11b/Mac-1a

Isotype: IgG1

Clone: ICRF44

Description: Human CD11b/Mac-1 CD11b is the 165 kDa M chain which combines with the CD18 2 integrin subunit to form the Mac-1 heterodimer. It is expressed on activated lymphocytes, monocytes, granulocytes and a subset of NK cells. CD11b/Mac-1 functions in cell-cell and cell-substrate interactions and is a ligand for C3bi, CD50 (ICAM-3), CD54 (ICAM-1) and CD102 (ICAM-2).

Application Details

Application Notes: Working Dilutions: Flow Cytometry: Purified (UNLB) (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (2 L/10^6 cells), Allophycocyanin (2 L/10^6 cells), Spectral RedTM (2 L/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Storage: The purified (UNLB) antibody is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of phosphate buffered saline containing 0.1% PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.1 mL in 0.2 mL of phosphate buffered saline containing 0.1% PBS/NaN3. Store at 2-8C.. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The Spectral RedTM (SPRD) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed. WARNING Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Restrictions: For Research Use only

Publications

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994 (PubMed).

Lee, Bankston, Arnaout et al.: "Two conformations of the integrin A-domain (I-domain): a pathway for activation?" in: Structure (London, England : 1993), Vol. 3, Issue 12, pp. 1333-40, 1996 (PubMed).