CD45 antibody (SPRD)

Catalog No. ABIN135780

The SPRD-conjugated Mouse Monoclonal anti-CD45 antibody (Clone F10-89-4) (ABIN135780) specifically detects CD45 in FACS. The antibody is reactive with Human samples.

Quick Overview for CD45 antibody (SPRD) (ABIN135780)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45 antibody is conjugated to SPRD

Application: Flow Cytometry (FACS)

Clone: F10-89-4

Product Details anti-CD45 Antibody (SPRD)

Specificity: Human/Horse CD45, Mr 180-220 kDa

Characteristics: Mouse Anti-Human CD45-SPRD

Immunogen: Purified T cells from human lymph nodes

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , WB - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, PE, PE/TXRD, APC, SPRD, CY5, PE/CY5.5, 10 L/106 cells PE/CY7, and AF700 conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Buffer: 100 tests in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45

Background: CD45, also known as the leukocyte common antigen (LCA), exists as four different isoforms (180, 190, 205, 220 kDa) that are generated by alternative splicing of three exons. LCA is found on all cells of hematopoietic origin, except erythrocytes and platelets. CD45 expression is required for T cell and B cell antigen receptor-mediated activation.

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling