alpha Fetoprotein antibody (AA 501-609)

Catalog No. ABIN1385277

Product Details anti-alpha Fetoprotein Antibody

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Binding Specificity: AA 501-609

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Alpha Fetoprotein

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-alpha Fetoprotein antibody (ABIN1385277)

Mo, He, Zhou, Ya, Feng, Yu, Deng: "A novel ECL sensor based on a boronate affinity molecular imprinting technique and functionalized SiO2@CQDs/AuNPs/MPBA nanocomposites for sensitive determination of alpha-fetoprotein." in: Biosensors & bioelectronics, Vol. 126, pp. 558-564, (2019) (PubMed).

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP (AFP Products)

Synonyms: FETA antibody, HPAFP antibody, alpha fetoprotein antibody, alpha-fetoprotein antibody, AFP antibody, Afp antibody

Background:

Synonyms: Alpha Fetoprotein, Alpha-fetoprotein, AFP, alpha 1 fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, alpha fetoglobulin, alpha Fetoprotein, Alpha fetoprotein precursor, FETA, HPAFP, FETA_HUMAN.

Background: Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

Gene ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process