UNC5A antibody (AA 125-220) (Alexa Fluor 350)

Catalog No. ABIN1391601

Product Details anti-UNC5A Antibody (Alexa Fluor 350)

Target: UNC5A (Unc-5 Homolog A (UNC5A))

Binding Specificity: AA 125-220

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UNC5A antibody is conjugated to Alexa Fluor 350

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Unc5a

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for UNC5A

Target: UNC5A (Unc-5 Homolog A (UNC5A))

Alternative Name: Unc5a/UNC5H1 (UNC5A Products)

Synonyms: zgc:175190 antibody, UNC5H1 antibody, Unc5h1 antibody, mKIAA1976 antibody, unc-5 netrin receptor A antibody, netrin receptor UNC5A antibody, UNC5A antibody, Tsp_02512 antibody, Tsp_13881 antibody, unc5a antibody, Unc5a antibody

Background:

Synonyms: Netrin receptor UNC 5A, Netrin receptor UNC5A, Netrin receptor Unc5h1, Protein unc-5 homolog 1, Protein unc-5 homolog A, Unc 5 homolog 1, Unc 5 homolog A, Unc 5a, Unc5 a, Unc5 homolog 1, Unc5 homolog A, Unc5a, UNC5A_HUMAN, Unc5h1, FLJ16449, KIAA1976.

Background: The UNC5H family of proteins act as transmembrane receptors for netrin-1 and play a crucial role in axon guidance and migration of neural cells. Additionally, when cleaved by a caspase to produce an intracellular fragment containing a death domain,UNC5H receptors induce apoptosis. This activity is blocked by the binding of netrin-1. In the absence of netrin-1, UNC5H receptors act as tumor suppressors by inhibiting anchorage-independent growth and invasion, but mutation of these receptors provides a potential mechanism for tumorigenicity. The expression of UNC5H receptors is downregulated in multiple carcinomas, including colorectal, breast, ovary, uterus, stomach, lung and kidney cancers. UNC5H1, also known as UNC5HA (unc-5 homolog A), is a member of the UNC5H family of proteins and is localized to the cell membrane. Three isoforms of UNC5H1 exist due to alternative splicing events.