FICD antibody (AA 161-250) (HRP)

Catalog No. ABIN1410704

Product Details anti-FICD Antibody (HRP)

Target: FICD (FIC Domain Containing (FICD))

Binding Specificity: AA 161-250

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FICD antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HYPE

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FICD

Target: FICD (FIC Domain Containing (FICD))

Alternative Name: HYPE/HIP13 (FICD Products)

Synonyms: HIP13 antibody, HYPE antibody, UNQ3041 antibody, D5Ertd40e antibody, Hype antibody, FIC domain containing antibody, FICD antibody, Ficd antibody

Background:

Synonyms: Adenosine monophosphate-protein transferase FICD, AMPylator FICD, FIC domain containing, FIC domain containing protein, FIC domain-containing protein, Fic S phase protein cell division homolog, ficd, FICD_HUMAN, HIP-13, HIP13, Huntingtin interacting protein 13, Huntingtin interacting protein E, Huntingtin interactor protein E, Huntingtin yeast partner E, Huntingtin-interacting protein 13, Huntingtin-interacting protein E.

Background: Huntingtin yeast partner E is a 458 amino acid single-pass membrane protein. HYPE is thought to interact with Huntingtin, a protein which induces neurodegeneration when mutated. HYPE also contains two tetratricopeptide repeats (TPR), which may be involved in protein-protein interaction. The gene that encodes HYPE is located on chromosome 12, which encodes over 1,100 genes within 132 million bases and makes up about 4.5 % of the human genome. A number of skeletal deformities are linked to chromosome 12 including hypochondrogenesis, achondrogenesis and Kniest dysplasia. Chromosome 12 is also home to a homeobox gene cluster which encodes crucial transcription factors for morphogenesis, and the natural killer complex gene cluster encoding C-type lectin proteins which mediate the NK cell response to MHC I interaction. Trisomy 12p leads to facial development defects, seizure disorders and a host of other symptoms varying in severity depending on the extent of mosaicism and is most severe in cases of complete trisomy.