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Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term), (pSer12) antibody

Details for Product No. ABIN1449623, Supplier: Log in to see
Antigen
  • MGC69006
  • MGC89867
  • MAP1LC3A
  • map1lc3a
  • Anx3
  • LC3
  • LRRGT00047
  • ATG8E
  • LC3A
  • MAP1ALC3
  • MAP1BLC3
  • 1010001H21Rik
  • 4922501H04Rik
  • LC3a
  • zgc:77094
  • Map1lc3
  • zgc:56434
  • wu:fb60g11
  • Lc3
  • b3gnt5
  • fb12f12
  • wu:fb12f12
  • wu:fe50g08
  • microtubule-associated protein 1 light chain 3 alpha
  • annexin A3
  • microtubule-associated protein 1 light chain 3 beta
  • UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5a
  • map1lc3a
  • MAP1LC3A
  • Anxa3
  • Map1lc3a
  • map1lc3b
  • b3gnt5a
Epitope
AA 1-30, pSer12, N-Term
70
36
25
24
21
20
18
17
14
13
13
13
13
13
12
12
10
8
7
7
6
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
380
190
162
35
25
17
17
16
14
13
12
8
6
5
4
3
3
2
1
1
1
1
Host
Rabbit
336
35
5
4
Clonality
Polyclonal
Conjugate
Un-conjugated
25
23
21
20
17
17
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), Enzyme Immunoassay (EIA), Western Blotting (WB)
310
162
135
106
70
61
31
27
17
15
14
10
5
3
2
1
Options
Supplier
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Immunogen KLH conjugated synthetic phosphopeptide between 1-30 amino acids surrounding Ser12 of Human LC3
Isotype Ig
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Bovine.
Species reactivity (tested):Human.
Purification Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name LC3 (MAP1LC3A Antibody Abstract)
Background MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).Synonyms: Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3A, MAP1 light chain 3-like protein 1, MAP1A / 1B light chain 3 A, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1LC3A, Microtubule-associated protein 1 light chain 3 alpha, Microtubule-associated proteins 1A/1B light chain 3A
Molecular Weight 14272 Da
NCBI Accession NM_032514
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer PBS, 0.09 % (W/V) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Dot Blot (DB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term), (pSer12) antibody (ABIN1449623) Dot blot analysis of Phospho-LC3 (APG8a) - Ser12 Antibody and Non phospho-LC3 (APG8a)...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term), (pSer12) antibody (ABIN1449623) Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term), (pSer12) antibody (ABIN1449623) Immunoblots of SH-SY5Y cells treated with MPP+ for 24h was probed with AP32191PU-N. T...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term), (pSer12) antibody (ABIN1449623) Immunoblots of SH-SY5Y cells treated with rapamycin for 1 h was probed with AP32191PU...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term), (pSer12) antibody (ABIN1449623) Something like SH-SY5Y cells expressing GFP-LC3-WT or-S12D treated with rapamycin or ...
Product cited in: Su, Chao, Huang, Weng, Jeng, Lai: "Rab5 and class III phosphoinositide 3-kinase Vps34 are involved in hepatitis C virus NS4B-induced autophagy." in: Journal of virology, Vol. 85, Issue 20, pp. 10561-71, 2011 (PubMed).

Background publications Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Tanida, Sou, Ezaki, Minematsu-Ikeguchi, Ueno, Kominami: "HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36268-76, 2004 (PubMed).

He, Dang, Dai, Guo, Wu, She, Pei, Chen, Ling, Wu, Zhao, Liu, Yu: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).