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ATG4B antibody (ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae)) (AA 23-53)

Details for Product anti-ATG4B Antibody No. ABIN1449637, Supplier: Log in to see
Antigen
  • 2510009N07Rik
  • Apg4b
  • APG4B
  • apg4b
  • Atg4bl
  • Aut2b2
  • Autl1
  • AUTL1
  • AW048066
Epitope
AA 23-53, N-Term
22
22
16
16
13
13
13
8
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
139
51
34
5
4
4
4
4
3
2
2
1
1
1
Host
Rabbit
134
7
1
Clonality
Polyclonal
Conjugate
This ATG4B antibody is un-conjugated
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
115
50
50
22
11
5
2
2
1
1
1
Supplier
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Immunogen KLH conjugated synthetic peptide between 23~53 amino acids from the N-terminal region of Human APG4B Genename: ATG4B
Isotype Ig
Purification Saturated Ammonium Sulfate precipitation followed by dialysis against PBS
Alternative Name APG4B / ATG4B (ATG4B Antibody Abstract)
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.Synonyms: AUT-like 1 cysteine endopeptidase, AUTL1, Autophagin-1, Autophagy-related cysteine endopeptidase 1, Autophagy-related protein 4 homolog B, Cysteine protease ATG4B, KIAA0943
Molecular Weight 44309 Da
Gene ID 23192
NCBI Accession NP_037457
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ATG4B antibody (ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae)) (AA 23-53) (ABIN1449637) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-ATG4B antibody (ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae)) (AA 23-53) (ABIN1449637) APG4B / ATG4B Antibody used in Western blot to detect APG4B in CEM tissue lysate.
Western Blotting (WB) image for anti-ATG4B antibody (ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae)) (AA 23-53) (ABIN1449637) Western blot analysis of APG4B / ATG4B Antibody  in 293 cell line lysates transiently...
Western Blotting (WB) image for anti-ATG4B antibody (ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae)) (AA 23-53) (ABIN1449637) Western blot analysis of APG4B / ATG4B Antibody  in mouse cerebellum tissue lysates (...
Background publications Garneau, Alvarez, Paquin, Lussier, Rancourt, Tremblay, Beaulieu, Rivard: "Nuclear expression of E2F4 induces cell death via multiple pathways in normal human intestinal epithelial crypt cells but not in colon cancer cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 293, Issue 4, pp. G758-72, 2007

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004