Histone H2B antibody (acLys12)

Catalog No. ABIN1535516

This anti-Histone H2B antibody is a Rabbit Polyclonal antibody detecting Histone H2B in WB, ELISA, IHC and IF. Suitable for Human and Mouse.

Quick Overview for Histone H2B antibody (acLys12) (ABIN1535516)

Target: Histone H2B

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Histone H2B Antibody

Binding Specificity: AA 10-59, acLys12

Specificity:

Histone H2B (Acetyl-Lys12) Antibody detects endogenous levels of total Histone H2B protein only when acetylated at Lys12.

AcetylationH:K12 M:K12

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys12.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

Comment:

Unigene-Number: Hs.473961 (NCBI Gene Symbol: H2BFS)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Histone H2B

Target: Histone H2B

Background:

Synonyms: Histone cluster 1, H2bb,H2B.1, H2B/f, H2BFF, H2B histone family, member F,histone 1, H2bb

NCBI Gene Symbol: H2BFS

Molecular Weight: 13 kDa

Gene ID: 54145

UniProt: P57053