Histone H2B antibody

Catalog No. ABIN7204986

The Mouse Monoclonal anti-Histone H2B antibody is suitable to detect Histone H2B in samples from Human, Mouse and Rat. It has been validated for WB and IF.

Quick Overview for Histone H2B antibody (ABIN7204986)

Target: Histone H2B

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Histone H2B Antibody

Purpose: Mouse Anti-Histone H2B Monoclonal Antibody

Specificity: The antibody detects endogenous Histone H2B protein.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: Synthetic Peptide

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-3000,IF 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.5 % BSA, 0.02 % sodium azide as Preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Histone H2B

Target: Histone H2B

Background: HIST1H2BB, H2BFF, Histone H2B type 1-B, Histone H2B.1, Histone H2B.f, H2B/fHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST1H2BB is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from HIST1H2BB lack polyA tails, instead, they contain a palindromic termination element. HIST1H2BB is found in the large histone gene cluster on chromosome 6p22-p21.3.

Molecular Weight: 14kD

Gene ID: 3018

UniProt: P33778