WNT1 antibody

Catalog No. ABIN155191

Product Details anti-WNT1 Antibody

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: The antibody recognizes Human WNT-1. Other species not tested.

Purification: Affinity chromatography employing immobilized hWNT-1 matrix

Immunogen: Highly pure (>98%) recombinant human WNT-1

Application Details

Application Notes: ElisaIndirect: To detect hWNT-1 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hWNT-1. Sandwich: To detect hWNT-1 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human WNT-1 (PP1231B) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1. Western BlotTo detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable for one year from despatch at -20 °C. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 12 months

Target Details for WNT1

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Alternative Name: WNT1 (WNT1 Products)

Synonyms: Xint-1 antibody, Xwnt1 antibody, int-1 antibody, int1 antibody, wnt-1 antibody, wnt1-a antibody, Int-1 antibody, Wnt-1 antibody, sw antibody, swaying antibody, BMND16 antibody, INT1 antibody, OI15 antibody, Int1 antibody, WNT-1 antibody, sb:eu647 antibody, zgc:194464 antibody, zgc:194478 antibody, WNT1 antibody, Wnt family member 1 antibody, Wnt family member 1 L homeolog antibody, wingless-type MMTV integration site family, member 1 antibody, WNT1 antibody, wnt1.L antibody, Wnt1 antibody, wnt1 antibody

Background: Synonyms: INT-1, INT1, Proto-oncogene protein Wnt-1

Gene ID: 7471

NCBI Accession: NP_005421

UniProt: P04628

Pathways: WNT Signaling, Dopaminergic Neurogenesis