DZIP3 antibody (AA 451-550) (HRP)

Catalog No. ABIN1712040

Product Details anti-DZIP3 Antibody (HRP)

Target: DZIP3 (DAZ Interacting Protein 3, Zinc Finger (DZIP3))

Binding Specificity: AA 451-550

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DZIP3 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DZIP3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DZIP3

Target: DZIP3 (DAZ Interacting Protein 3, Zinc Finger (DZIP3))

Alternative Name: DZIP3 (DZIP3 Products)

Synonyms: 2310047C04Rik antibody, 2A-HUB antibody, 6430549P11Rik antibody, A230104G20 antibody, PPP1R66 antibody, UURF2 antibody, hRUL138 antibody, RGD1563278 antibody, DAZ interacting protein 3, zinc finger antibody, DAZ interacting zinc finger protein 3 antibody, Dzip3 antibody, DZIP3 antibody

Background:

Synonyms: DAZ-interacting protein 3, E3 ubiquitin-protein ligase DZIP3, hRUL138, KIAA0675, RNA-binding ubiquitin ligase of 138 kDa, DZIP3_HUMAN.

Background: Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). DZIP3 (DAZ interacting protein 3, zinc finger), also known as UURF2 or hRUL138, is a 1,208 amino acid protein that localizes to the cytoplasm and contains one RING-type zinc finger. Expressed in a variety of tissues with highest expression in heart, skeletal muscle and kidney, DZIP3 functions as an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme, thereby playing a role in signaling events throughout the cell. Multiple isoforms of DZIP3 exist due to alternative splicing events.

Gene ID: 9666