GNPDA1 antibody (AA 201-289) (HRP)

Catalog No. ABIN1712543

Product Details anti-GNPDA1 Antibody (HRP)

Target: GNPDA1 (Glucosamine-6-Phosphate Deaminase 1 (GNPDA1))

Binding Specificity: AA 201-289

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNPDA1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GNPDA1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GNPDA1

Target: GNPDA1 (Glucosamine-6-Phosphate Deaminase 1 (GNPDA1))

Alternative Name: GNPDA1 (GNPDA1 Products)

Synonyms: GNP1 antibody, GNPDA antibody, GNPI antibody, GPI antibody, HLN antibody, Gnp1 antibody, Gnpi antibody, oscillin antibody, gnpda antibody, gnpi antibody, gpi antibody, hln antibody, GNPDA1 antibody, zgc:110691 antibody, glucosamine-6-phosphate deaminase 1 antibody, glucosamine-6-phosphate deaminase 1 S homeolog antibody, Glucosamine-6-phosphate deaminase 1 antibody, GNPDA1 antibody, Gnpda1 antibody, gnpda1.S antibody, gnpda1 antibody, MSMEG_0501 antibody, nagB antibody, PPSC2_c4268 antibody

Background:

Synonyms: EC 3.5.99.6, GlcN6P deaminase 1, Glucosamine 6 phosphate deaminase 1, Glucosamine 6 phosphate isomerase 1, Glucosamine-6-phosphate deaminase 1, Glucosamine-6-phosphate isomerase 1, GNPDA 1, GNPDA1, GNPI, GNPI1_HUMAN, HLN, KIAA0060, Oscillin, Putative glucosamine 6 phosphate isomerase.

Background: During fertilization in mammals, the sperm activates the egg by causing an increase in the level of free cytoplasmic calcium concentration. This increased calcium concentration induces a characteristic series of oscillations that trigger egg activation and early embryo development. A hamster protein named oscillin is thought to be involved in this pathway. The enzyme glucosamine-6-phosphate isomerase (GNPI) or deaminase (GNPDA1) and the related protein GNPDA2 are the human homologs of hamster oscillin. GNPDA1 and GNPDA2 catalyze the conversion of GNP to fructose-6-phosphate and ammonia. Both proteins exist as homohexamers and are ubiquitously expressed with highest expression in testis, ovary and heart. Three isoforms of GNPDA2 are expressed due to alternative splicing events.

Gene ID: 10007