Rab3c antibody (AA 154-227)

Catalog No. ABIN1742212

Product Details anti-Rab3c Antibody

Target: Rab3c (RAB3C, Member RAS Oncogene Family (Rab3c))

Binding Specificity: AA 154-227

Reactivity: Human, Mouse, Rat, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rab3c antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Specific for Rab 3c.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant protein of human Rab 3c (aa 154-227).

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC: not tested yet

Comment:

This antibody has been affinity purified with a peptide consisting only of the Rab 3c specific epitope NTRLKETPPPPQPN (aa 211 - 224) of the human protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-Rab3c antibody (ABIN1742212)

Nishioka, Tooi, Isobe, Nakatsuji, Aiba: "BMS-708163 and Nilotinib restore synaptic dysfunction in human embryonic stem cell-derived Alzheimer's disease models." in: Scientific reports, Vol. 6, pp. 33427, (2016) (PubMed).

Pavlos, Grønborg, Riedel, Chua, Boyken, Kloepper, Urlaub, Rizzoli, Jahn: "Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 40, pp. 13441-53, (2010) (PubMed).

Cai, Reim, Varoqueaux, Tapechum, Hill, Sørensen, Brose, Chow: "Complexin II plays a positive role in Ca2+-triggered exocytosis by facilitating vesicle priming." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 49, pp. 19538-43, (2008) (PubMed).

Target Details for Rab3c

Target: Rab3c (RAB3C, Member RAS Oncogene Family (Rab3c))

Alternative Name: Rab 3c (Rab3c Products)

Synonyms: 2700062I01Rik antibody, 3110015B08Rik antibody, 3110037E15Rik antibody, AI850886 antibody, rab3d antibody, MGC130815 antibody, zgc:92916 antibody, RAB3C antibody, RAB3C, member RAS oncogene family antibody, RAB3D, member RAS oncogene family L homeolog antibody, Rab3c antibody, RAB3C antibody, rab3d.L antibody, rab3c antibody