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BU-1A / B6.1 antibody (FITC)

Reactivity: Chicken FACS Host: Mouse Monoclonal L22 FITC
Catalog No. ABIN180731
  • Target See all BU-1A / B6.1 products
    BU-1A / B6.1
    Reactivity
    • 12
    • 1
    Chicken
    Host
    • 12
    Mouse
    Clonality
    • 12
    Monoclonal
    Conjugate
    • 5
    • 3
    • 2
    • 2
    This BU-1A / B6.1 antibody is conjugated to FITC
    Application
    • 12
    • 7
    • 7
    • 4
    Flow Cytometry (FACS)
    Specificity
    MCA2170F recognises the 'a' allele of the polymorphic cell surface antigen Bu-1.
    Characteristics
    Synonyms: B-Cell Marker, BU-1 Allele A
    Purification
    Affinity chromatography on Protein G
    Immunogen
    Chicken Bursa cellsGenename: BU-1A
    Clone
    L22
    Isotype
    IgG1
  • Application Notes
    Flow Cytometry: 1/20 - 1/80, Use 10 μL of the suggested working dilution to label 106 cells in100 μL. Method sheets are available on request.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    BPS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store at +4 °C or at -20 °C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Should this productcontain a precipitate we recommend microcentrifugation before use.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    BU-1A / B6.1
    Abstract
    BU-1A / B6.1 Products
    Synonyms
    AL022991 antibody, Bub1a antibody, C80208 antibody, D2Xrf87 antibody, BUB1, mitotic checkpoint serine/threonine kinase antibody, BUB1 mitotic checkpoint serine/threonine kinase antibody, Bub1 antibody
    Background
    Bu-1a is a 70kD alloantigen expressed on B cells and a subset of macrophages. Reports suggest that Bu-1 is expressed on B cell precursors at early stages of development. Weak cross-reactivity with Bu-1b may be seen at higher antibody levels. This may be removed by appropriate titration. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line.Synonyms: B-Cell Marker, BU-1 Allele A
    Gene ID
    396098
    UniProt
    Q90746
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