Podoplanin antibody

Catalog No. ABIN180933

The Mouse Monoclonal anti-Podoplanin antibody (Clone 4D5aE5E6) (ABIN180933) specifically detects Podoplanin in IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for Podoplanin antibody (ABIN180933)

Target: Podoplanin (PDPN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 4D5aE5E6

Product Details anti-Podoplanin Antibody

Specificity: This antibody recognizes Human Podoplanin (gp36).

Characteristics: Synonyms: Glycoprotein 36, PA2.26 antigen, T1-alpha, Aggrus, PDPN, GP36, PSEC0003, PSEC0025

Purification: Affinity chromatography on Protein G

Immunogen: ORF Human podoplanin ectodomain expressed in E. coliPage 1 of 3.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections: 0.75g/mL - 2g/mL. Immunohistochemistry on paraffin sections: This product requires antigen retrieval usingheat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 isrecommended for this purpose. Recommended Positive Control: Normal lung
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS containing 0,09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin

Background: Podoplanin a 36 kDa type I cell membrane-associated glycoprotein, with a broad spectrum of distribution, being strongly expressed on the lymphatic endothelium found in lung, placenta and skeletal muscle and to a lesser extent in brain, kidney and liver. Podoplanin is absent on blood endothelium and therefore This antibody is readily able to distinguish between lymphatic vessels and blood vasculature. Although the specific function of this protein is undetermined, Podoplanin is reported to be a reliable indicator of tumour lymphangiogenesis.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha

Gene ID: 10630

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport