CCL26 antibody

Catalog No. ABIN181588

The Goat Polyclonal anti-CCL26 antibody (ABIN181588) specifically detects CCL26 in WB, Func and EIA. The antibody is reactive with Human samples.

Quick Overview for CCL26 antibody (ABIN181588)

Target: CCL26 (Chemokine (C-C Motif) Ligand 26 (CCL26))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL26 antibody is un-conjugated

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Product Details anti-CCL26 Antibody

Specificity: This antibody detects Eotaxin-3.

Purification: Immunoaffinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human Eotaxin-3

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhEotaxin-3 (100 ng/mL), a concentration of 5.0 - 7.0 μg/mL of this antibody is required. ELISA: Indirect: To detect hEotaxin-3 by indirect ELISA (using 100 μL/well antibody solution)a concentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hEotaxin-3. Sandwich: To detect hEotaxin-3 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with Biotinylated Anti-HumanEotaxin-3 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hEotaxin-3. Western Blot: To detect hEotaxin-3 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hEotaxin-3 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for CCL26

Target: CCL26 (Chemokine (C-C Motif) Ligand 26 (CCL26))

Alternative Name: Eotaxin-3 / CCL26

Background: Eotaxin 3 is a recently discovered CC chemokine. It binds to the CCR3 receptor and is highly chemotactic for eosinophils and basophils.Synonyms: C-C motif chemokine 26, CC chemokine IMAC, IMAC, MIP4 alpha, Macrophage inflammatory protein 4-alpha, SCYA26, Small-inducible cytokine A26, TSC-1, TSC1, Thymic stroma chemokine-1

Gene ID: 10344

NCBI Accession: NP_006063

UniProt: Q9Y258

Pathways: Regulation of Actin Filament Polymerization