Add to Basket
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

CD4 Molecule (CD4) antibody

Antigen

CD4 Molecule (CD4)
Synonyms CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25
Clonality Monoclonal (RPA-T4)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
Catalog no. ABIN181799
Quantity 0.1 mg  (1 mg/ml)  (Variants)
Price 190.00 $   Plus shipping costs $35.00
Shipping to
Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name CD4
Swiss-Prot P01730
Immunogen Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
Isotype IgG1  (Matching secondary antibodies)
Clone RPA-T4
Description CD4 is a 55kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages.
Specificity This antibody recognises CD4. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognise an epitope within domain 1, of the extracellular region, of the CD4 molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation. We recommend the use of ABIN118486 for this purpose.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Flow Cytometry: 1/50 - 1/100, Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood. Immunohistochemistry on frozen sections: 1/10 - 1/100. Recommended Positive Control: Human lymphatic tissue Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 1 mg/ml
Buffer PBS, pH 7.4 containing 0.09% Sodium Azide
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "CD4 Molecule (CD4)", type "Antibodies"
Hosts Mouse (444), Rat (208), Rabbit (68)
Reactivities Human (320), Mouse (Murine) (239), Rat (Rattus) (106), Dog (Canine) (44), Chicken (34), Cow (Bovine) (34), Sheep (Ovine) (24), Pig (Porcine) (22), Cat (Feline) (20), Rabbit (20), Bird (Avian) (8), Guinea Pig (8), Horse (Equine) (4), Duck (3), Monkey (3)
Applications Flow Cytometry (FACS) (601), Immunohistochemistry (Frozen Sections) (IHC (fro)) (148), Immunoprecipitation (IP) (93), Western Blotting (WB) (83), Immunofluorescence (IF) (53), ELISA (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (49), Functional Studies (Func) (35), Immunohistochemistry (IHC) (29), Immunocytochemistry (ICC) (16), Blocking Antibody (Inhibition) (11), Cytotoxicity Test (CyTox) (11), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Neutralization (Neut) (1)
Conjugates FITC (116), PE (77), Biotin (71), APC (40), RPE (22), Cy5 (9), Cy5,PE (9), Cy5.5 (6), Cy7,PE (6), Alexa Fluor 488 (5), PE,Cy7 (5), HRP (4), PE,Cy5.5 (4), Pacific Blue (4), APC,Cy7 (3), PE,Cy5 (3), PerCP (3), SPRD (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Alexa Fluor 700 (2), Cy3 (2), Cy7 (2), Gold (2), PE,Cy3 (2), RBITC (2), DY647 (1), Dyomics 647 (1), PE,DY590 (1), PE,DY647 (1), PE,Texas Red (TR) (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP,Cy5.5 (1), RPE,Cy5 (1), RPE,Cy7 (1)
Epitopes domain 1 (10), domain 2 (5), non-polymorphic (5)