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CD4 antibody (PE)

CD4 Reactivity: Dog FACS Host: Rat Monoclonal YKIX302-9 PE

Catalog No. ABIN182021

Datasheet as PDF

Target See all CD4 Antibodies

CD4
Reactivity
334

208

119

48

24

20

19

18

17

10

10

10

10

10

10

9

9

9

8

5

4

2

1

1

1

1

1
Dog
Host
349

147

112

3

3

1
Rat
Compatible Secondaries
Clonality
469

138

5
Monoclonal
Conjugate
205

69

61

44

34

12

11

10

9

9

9

9

8

8

7

5

5

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1
This CD4 antibody is conjugated to PE
Application
493

191

127

102

93

69

69

42

39

34

31

29

10

8

8

7

6

6

5

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS)

Specificity

This antibody recognises the CD4 cell surface antigen. The antibody has been shown to block MHCII dependent T cell responses in vivo and in vitro.

Purification

Affinity Chromatography on Protein G

Immunogen

Canine Concanavilin A activated T-cell blasts. Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line

Clone

YKIX302-9

Isotype

IgG2a

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anti-CD4 (CD4) (AA 385-457) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Pig, Cat, Cow, Rabbit, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB, IHC Host: Goat Polyclonal unconjugated

anti-CD4 (CD4) (AA 231-330) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody
CD4 Reactivity: Human FACS, WB, CyTOF Host: Mouse Monoclonal MEM-241 unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Mouse FACS, IP, IHC (fro), ICC, Func Host: Rat Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (AA 301-400) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE

anti-CD4 (CD4) antibody (PE)
CD4 Reactivity: Rat FACS Host: Mouse Monoclonal OX-35 PE

anti-CD4 (CD4) (AA 26-200) antibody
CD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Flow Cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Reconstitution

Restore with 1.0 mL distilled water

Concentration

0.1 mg/mL

Buffer

PBS, 0.09 % Sodium Azide, 1 % BSA

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf Life: one year from despatch.Reconstitution: Restore with 1.0 ml distilled water.

Expiry Date

12 months
Target

CD4

Alternative Name

CD4 (CD4 Products)

Background

In the dog CD4 is expressed by neutrophils as well as by lymphocytes subsets.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene ID

403931

Pathways

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events