EEA1 antibody (N-Term)

Catalog No. ABIN182326

This Rabbit Polyclonal antibody specifically detects EEA1 in WB, IHC and IP. It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit, Horse, Cow and Guinea Pigand has been mentioned in 1 publication.

Quick Overview for EEA1 antibody (N-Term) (ABIN182326)

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Horse, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-EEA1 Antibody

Binding Specificity: N-Term

Sequence: LTENLLKKEQ DYTKLEEKHN EESVSKKNIQ ATLHQKDLDC QQLQSRLSAS

Predicted Reactivity: Cow: 92%, Dog: 75%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 92%, Rabbit: 85%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against EEA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EEA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1411 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EEA1 antibody (ABIN182326)

Cebrian, Croce, Guerrero, Blanchard, Mayorga: "Rab22a controls MHC-I intracellular trafficking and antigen cross-presentation by dendritic cells." in: EMBO reports, Vol. 17, Issue 12, pp. 1753-1765, (2016) (PubMed).

Target Details for EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Alternative Name: EEA1

Background: EEA1 is involved in neuronal synaptic vesicle function and axonal transport and growth. EEA1 may undergo calcium-dependent conformational changes that are required for binding to SNAP-25.
Alias Symbols: MST105, ZFYVE2, MSTP105
Protein Interaction Partner: UBC, RNF2, BMI1, CFTR, ERBB2, VCP, EEA1, RAB5A, TNK2, RABEP1, RABGEF1, PDCD6IP, PXK, HDAC6, ZFYVE16, USO1, STX6, RAB22A, STX12, TOM1, RAB5C, MAPK14, RAB5B, IGF1R, NSF,
Protein Size: 1411

Molecular Weight: 162 kDa

Gene ID: 8411

NCBI Accession: NM_003566, NP_003557

UniProt: Q15075

Pathways: Toll-Like Receptors Cascades