EEA1 antibody (C-Term)

Catalog No. ABIN1439993

The Goat Polyclonal anti-EEA1 antibody is suitable to detect EEA1 in samples from Human, Mouse, Rat, Dog, Hamster, Rabbit, Horse, Monkey, Pig, Cow, Guinea Pig, Chicken, Avian, Cat, Donkey, Goat and Sheep. It has been validated for WB, IF and IHC (fro).

Quick Overview for EEA1 antibody (C-Term) (ABIN1439993)

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Reactivity: Human, Mouse, Rat, Dog, Hamster, Rabbit, Horse, Monkey, Pig, Cow, Guinea Pig, Chicken, Avian, Cat, Donkey, Goat, Sheep

Host: Goat

Clonality: Polyclonal

Conjugate: This EEA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-EEA1 Antibody

Binding Specificity: C-Term

Purpose: Anti-EEA1

Sequence: KKHEENEAKL TMQITALNEN LGTVKKEWQS SQRRVSELEK QTDDLRGEIA VLEATVQNNQ DERRALLERC LKGEGEIEKL QTKVLELQRK LDNTTAAVQE LGRENQSLQI KHTQALNRKW AEDNEVQNCM ACGKGFSVTV RRHHCRQCGN IFCAECSAKN ALTPSSKKPV RVCDACFNDL QG

Specificity: Detects a band of 180 kDa by Western blot in the following human, mouse and rat whole cell lysates.

Characteristics: Early Endosome Antigen 1

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide within residues 1230 aa to the C-terminus of human EEA1 produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:250-1:1,000, IF:1:25-1:250, IHC-F:1:100-1:500

Restrictions: For Research Use only

Handling

Concentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Alternative Name: EEA1

Background:

Goat polyclonal antibody to EEA1 - early endosome marker. EEA1 is Early Endosome Antigen 1 protein that binds phospholipid vesicles containing phosphatidylinositol 3-phosphate, which is necessary for endosomal trafficking.

Early endosome antigen 1, 162kD, early endosome-associated protein, endosome-associated protein p162, zinc finger FYVE domain-containing protein 2 antibody.

Pathways: Toll-Like Receptors Cascades