Sirtuin 5 (SIRT5) antibody
| Antigen |
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| Synonyms |
SIR2L5, FLJ36950, AV001953, 0610012J09Rik, 1500032M05Rik, MGC93823, zgc:92288, SIRT5, MGC128489, sir2l5, DKFZp468D0719 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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 Alternatives Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Zebrafish, Xenopus laevis, Chicken, Pig (Porcine)
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Conjugate
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Biotin (5), FITC (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
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Application
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Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
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| Catalog no. |
ABIN182559 |
| Quantity |
50 µg (Variants) |
| Price |
317.90 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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Alternative name
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SIRT5
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Gene ID
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SIRT5
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UniProt
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Q9NXA8
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Immunogen
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The immunogen for anti-SIRT5 antibody: synthetic peptide directed towards the C terminal of human SIRT5
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Sequence
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EVDRELAHCDLCLVVGTSSVVYPAAMFAPQVAARGVPVAE FNTETTPATN
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Cross-Reactivity
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Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
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Format
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Lyophilized
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Reconstitution
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Add 50 µl of distilled water. Final anti-SIRT5 antibody concentration is 1 mg/ml.
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Description
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SIRT5 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in two transcript variants.
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Characteristics
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Antigen Size: 299 amino acids
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Molecular Weight
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32kDa
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Application Notes
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WB Suggested Anti-SIRT5 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate.
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Purification
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Affinity Purified
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Buffer
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PBS
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Storage (Long Term)
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For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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Storage (Short Term)
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Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
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Research Area
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Chromatin and Nuclear Signaling, Proteases
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Restrictions
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For Research Use only
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Product
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Frye: "Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins." in: Biochemical and biophysical research communications, Vol. 273, Issue 2, pp. 793-8, 2000 (PubMed).
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Alternatives for antigen "Sirtuin 5 (SIRT5)", type "Antibodies"
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Hosts
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Rabbit (47), Mouse (12), Chicken (3), Sheep (1)
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Reactivities
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Human (52), Mouse (Murine) (26), Rat (Rattus) (25), Pig (Porcine) (3), Cow (Bovine) (2), Chicken (1), Dog (Canine) (1)
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Applications
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Western Blotting (WB) (42), Immunofluorescence (IF) (22), ELISA (21), Immunohistochemistry (IHC) (10), Flow Cytometry (FACS) (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Biotin (5), FITC (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
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Epitopes
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C-Term (8), AA 30-46 (2), Internal Region (2), AA 278-293 (1), AA 3-3 (1), AA 33-310 (1), Center (1), N-Term (1)
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2013 BioMed Central Research Awards
