SIRT5 antibody (Middle Region)

Catalog No. ABIN2779613

This Rabbit Polyclonal antibody specifically detects SIRT5 in WB and IHC. It exhibits reactivity toward Human, Mouse, Cow, Goat, Rabbit and Pig.

Quick Overview for SIRT5 antibody (Middle Region) (ABIN2779613)

Target: SIRT5 (Sirtuin 5 (SIRT5))

Reactivity: Human, Mouse, Cow, Goat, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SIRT5 Antibody

Binding Specificity: Middle Region

Purpose: SIRT5 Antibody - middle region

Sequence: LSGKGAPEPG TQDASIPVEK LPRCEEAGCG GLLRPHVVWF GENLDPAILE

Predicted Reactivity: Cow: 77%, Goat: 77%, Human: 100%, Mouse: 77%, Pig: 85%, Rabbit: 85%

Characteristics: This is a rabbit polyclonal antibody against SIRT5. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SIRT5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32391-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SIRT5

Target: SIRT5 (Sirtuin 5 (SIRT5))

Alternative Name: SIRT5

Background:

Background Information: SIRT5 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family.

Gene Name: Sirtuin 5

Alternative Symbols: SIR2L5

Protein Name: NAD-dependent protein deacylase sirtuin-5, mitochondrial

Molecular Weight: 33kDa

Gene ID: 23408

NCBI Accession: NP_112534

UniProt: Q9NXA8

Pathways: SARS-CoV-2 Protein Interactome