UBE2N antibody (Middle Region)

Catalog No. ABIN182924

The Rabbit Polyclonal anti-UBE2N antibody has been validated for WB and IHC. It is suitable to detect UBE2N in samples from Human, Rat, Mouse, Zebrafish (Danio rerio), Dog, Cow, Sheep, Rabbit, Horse, Guinea Pig and Saccharomyces cerevisiae. There is 1 publication available.

Quick Overview for UBE2N antibody (Middle Region) (ABIN182924)

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Dog, Cow, Sheep, Rabbit, Horse, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2N antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-UBE2N Antibody

Binding Specificity: Middle Region

Sequence: VDKLGRICLD ILKDKWSPAL QIRTVLLSIQ ALLSAPNPDD PLANDVAEQW

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against UBE2N. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE2N

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 152 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-UBE2N antibody (ABIN182924)

Xu, Yan, Jovanovic, An, Cheng, Aakre, Yi, Eng, Link, Moses: "Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts." in: Proteomics, Vol. 10, Issue 13, pp. 2458-70, (2010) (PubMed).

Target Details for UBE2N

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Alternative Name: UBE2N

Background: UBE2N encodes a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
Alias Symbols: UBC13, UbcH-ben
Protein Interaction Partner: TRIM33, BFAR, MALT1, STUB1, BCL10, UBE2V2, UBC, TRAF6, ZNRF1, XIAP, SLX8, SLX5, MARCH7, TNF, UBE2N, RNF168, RNF8, PARK2, OTUB1, ARIH1, NEDD4L, MFN2, TRIM72, PELI3, RNF111, RNF11, BAG3, TRAF2, FN1, VCAM1, RNF126, RNF115, NBN, ATP6V1H, VPS33B, ZDHHC5, ACTR2
Protein Size: 152

Molecular Weight: 17 kDa

Gene ID: 7334

NCBI Accession: NM_003348, NP_003339

UniProt: P61088

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway