»
Antibodies
»
anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) antibody
Ubiquitin-Conjugating Enzyme E2N (UBE2N) antibody
| Antigen |
|
| Synonyms |
UBC13, AL022654, BB101821, 1500026J17Rik, MGC93937, ube2n, MGC64428, zgc:55726, wu:fb11b03, wu:fc08b06, UBE2N, MGC139855, ubc13, MGC75672, ubch-ben |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
 Alternatives Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Fruit Fly (Drosophila melanogaster), Zebrafish, Chicken, Xenopus laevis
|
|
Conjugate
|
Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
|
|
Application
|
|
| Catalog no. |
ABIN182924 |
| Quantity |
0.1 mg (Variants) |
| Price |
251.90 $ Plus shipping costs $45.00
|
|
Bulk discount
|
| Shipping to |
|
| Availability |
Will be delivered in 2 to 3 Business Days |
|
Alternative name
|
UBE2N / BLU
|
|
Gene ID
|
UBE2N
|
|
UniProt
|
P61088
|
|
Immunogen
|
The immunogen for anti-UBE2N antibody: synthetic peptide directed towards the middle region of human UBE2N
|
|
Sequence
|
VDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDD PLANDVAEQW
|
|
Cross-Reactivity
|
Dog (Canine), Chicken, Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus)
|
|
Format
|
Lyophilized
|
|
Reconstitution
|
Add 100 µl of distilled water. Final anti-UBE2N antibody concentration is 1 mg/ml.
|
|
Description
|
UBE2N encodes a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
|
|
Characteristics
|
Antigen Size: 152 amino acids
|
|
Molecular Weight
|
17kDa
|
|
Application Notes
|
WB Suggested Anti-UBE2N Antibody Titration: 1.25ug/ml
Positive Control: HepG2 cell lysate.
|
|
Purification
|
Protein A purified
|
|
Buffer
|
PBS
|
|
Storage (Long Term)
|
For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
|
|
Storage (Short Term)
|
Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
|
|
Restrictions
|
For Research Use only
|
|
Product
|
Bothos, Summers, Venere et al.: "The Chfr mitotic checkpoint protein functions with Ubc13-Mms2 to form Lys63-linked polyubiquitin chains." in: Oncogene, Vol. 22, Issue 46, pp. 7101-7, 2003 (PubMed).
|
Alternatives for antigen "Ubiquitin-Conjugating Enzyme E2N (UBE2N)", type "Antibodies"
|
Hosts
|
Rabbit (45), Goat (4), Mouse (2)
|
|
Reactivities
|
Human (44), Mouse (Murine) (24), Rat (Rattus) (24), Cow (Bovine) (20), Dog (Canine) (19), Chicken (18), Horse (Equine) (18), Rabbit (18), Fruit Fly (Drosophila melanogaster) (1), Zebrafish (1)
|
|
Applications
|
Western Blotting (WB) (35), ELISA (20), Immunofluorescence (IF) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Immunohistochemistry (IHC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (3), Immunoelectron Microscopy (IEM) (1)
|
|
Conjugates
|
Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
|
|
Epitopes
|
C-Term (6), N-Term (4), AA 40-51 (3), Internal Region (2), AA 2-19 (1)
|
2013 BioMed Central Research Awards
