Chromobox Homolog 3 (CBX3) antibody
| Antigen |
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| Synonyms |
MGC94711, Hp1-gamma, MGC105792, MGC105793, MGC156678, CBX3, HECH, HP1-GAMMA, HP1Hs-gamma |
| Clonality |
Polyclonal |
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Host
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Reactivity
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 Alternatives Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken, Xenopus laevis
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Conjugate
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2)
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Application
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Western Blotting (WB) (42), Immunofluorescence (IF) (40), ELISA (26), Immunohistochemistry (IHC) (17), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunoprecipitation (IP) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoelectron Microscopy (IEM) (2), Flow Cytometry (FACS) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN183071 |
| Quantity |
50 µg (Variants) |
| Price |
317.90 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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Alternative name
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CBX3
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Gene ID
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CBX3
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UniProt
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Q13185
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Immunogen
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The immunogen for anti-CBX3 antibody: synthetic peptide directed towards the middle region of human CBX3
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Sequence
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WEPEENLDCPELIEAFLNSQKAGKEKDGTKRKSLSDSESD DSKSKKKRDA
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Cross-Reactivity
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Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)
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Format
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Lyophilized
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Reconstitution
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Add 50 µl of distilled water. Final anti-CBX3 antibody concentration is 1 mg/ml.
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Description
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At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. CBX3 binds DNA and is a component of heterochromatin. CBX3 also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of CBX3 may explain the association of heterochromatin with the inner nuclear membrane.At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
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Characteristics
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Antigen Size: 183 amino acids
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Molecular Weight
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21kDa
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Application Notes
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WB Suggested Anti-CBX3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Jurkat cell lysate.
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Purification
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Affinity Purified
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Buffer
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PBS
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Storage (Long Term)
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For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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Storage (Short Term)
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Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
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Restrictions
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For Research Use only
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Product
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Scherer, Cheung, MacDonald et al.: "Human chromosome 7: DNA sequence and biology." in: Science (New York, N.Y.), Vol. 300, Issue 5620, pp. 767-72, 2003 (PubMed).
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Alternatives for antigen "Chromobox Homolog 3 (CBX3)", type "Antibodies"
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Hosts
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Rabbit (61), Goat (8), Mouse (5), Rat (2)
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Reactivities
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Human (70), Mouse (Murine) (54), Rat (Rattus) (49), Dog (Canine) (24), Chicken (20), Cow (Bovine) (20), Cat (Feline) (1), Fruit Fly (Drosophila melanogaster) (1), Xenopus laevis (1)
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Applications
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Western Blotting (WB) (42), Immunofluorescence (IF) (40), ELISA (26), Immunohistochemistry (IHC) (17), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunoprecipitation (IP) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoelectron Microscopy (IEM) (2), Flow Cytometry (FACS) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2)
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Epitopes
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pSer83 (19), Internal Region (4), AA 1-50 (1), AA 17-173 (1), AA 2-15 (1), AA 75-125 (1), C-Term (1), Center (1), Internal Region,AA 90-119 (1), N-Term (1), N-Term,AA 24-53 (1), pSer93 (1)
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