CLCN3 antibody (C-Term)

Catalog No. ABIN183185

The Rabbit Polyclonal anti-CLCN3 antibody has been validated for WB and IHC. It is suitable to detect CLCN3 in samples from Rat, Human, Mouse, Guinea Pig, Rabbit, Dog, Zebrafish (Danio rerio), Horse, Cow and Sheep. There is 1 publication available.

Quick Overview for CLCN3 antibody (C-Term) (ABIN183185)

Target: CLCN3 (Chloride Channel 3 (CLCN3))

Reactivity: Rat, Human, Mouse, Guinea Pig, Rabbit, Dog, Zebrafish (Danio rerio), Horse, Cow, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLCN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CLCN3 Antibody

Binding Specificity: C-Term

Sequence: VGDAFGREGI YEAHIRLNGY PFLDAKEEFT HTTLAADVMR PRRNDPPLAV L

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 91%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against CLCN3. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of human CLCN3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 866 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CLCN3 antibody (ABIN183185)

Jensen, Nosjean, Dziegiel, Boutin, Sørensen, Karsdal, Henriksen: "A quantitative assay for lysosomal acidification rates in human osteoclasts." in: Assay and drug development technologies, Vol. 9, Issue 2, pp. 157-64, (2011) (PubMed).

Target Details for CLCN3

Target: CLCN3 (Chloride Channel 3 (CLCN3))

Alternative Name: CLCN3

Background: CLCN3 plays an important role in the cell proliferation of vascular smooth muscle cells. The PDZ-binding isoform of CLCN3 resides in the Golgi where it co-localizes with a small amount of CFTR (cystic fibrosis transmembrane conductance regulator).
Alias Symbols: CLC3, ClC-3
Protein Interaction Partner: GOPC, SLC9A3R1, PDZK1, CLCN3, CFTR,
Protein Size: 866

Molecular Weight: 95 kDa

Gene ID: 1182

NCBI Accession: NM_173872, NP_776297

UniProt: P51790